fadH [2018-02-04T05:46:23.000Z]

2,4-dienoyl-CoA reductase

locus: BSU14060

pI: 6.49

mw: 26.98 kDa

protein length: 254 aa

gene length: 762 bp

function: fatty acid degradation

product: 2,4-dienoyl-CoA reductase

essential: no

ec: 1.3.1.34

synonyms: ykuF

Genomic Context

categories

• [category|SW 2|Metabolism] → [category|SW 2.4|Lipid metabolism] → [category|SW 2.4.1|Utilization of lipids] → [category|SW 2.4.1.3|Utilization of fatty acids]

Gene

Coordinates: 1,478,072 → 1,478,836

The protein

Catalyzed reaction/ biological activity

• Trans-2,3-didehydroacyl-CoA + NADP⁺ = trans,trans-2,3,4,5-tetradehydroacyl-CoA + NADPH (according to Swiss-Prot)

Protein family

• 2,4-dienoyl-CoA reductase subfamily (according to Swiss-Prot)

Paralogous protein(s)

• [protein|B6FF689E65906186F3576B378650D713DB84EDDA|YcdF], [protein|EAEEA4DD9641919830A81185333A2610B964D37C|YhdF], [protein|6047F2493FCE3D2A9BDB1AD88E5926ECEED81036|YhxC], [protein|4DEFC2998464BF8327578C36A13A10DD277F991E|YkvO]

Structure

• [PDB|3IMF] (from B. anthracis, 64% identity)

Expression and Regulation

Operons

• genes: [gene|CE990D33A12C50D22506A52E0099F7D97A762D4E|fadH]-[gene|0264C72F95D53F0211B52753DA37B4A8C0E982D2|fadG]-[gene|B46D407B116E358DFB6284466DE5926EB3719C53|ykzU]
  description: [Pubmed|17189250]

  regulatory mechanism

  • [protein|8A8F93BAD0582F712C9EE1C3FE6C04292E91F390|FadR]: repression, [Pubmed|17189250], in [regulon|8A8F93BAD0582F712C9EE1C3FE6C04292E91F390|FadR regulon]

  regulation

  • induced in the presence of long-chain fatty acids ([protein|8A8F93BAD0582F712C9EE1C3FE6C04292E91F390|FadR]) [Pubmed|17189250]
  • expression of the operon is strongly induced during [SW|biofilm formation] [pubmed|31113899]

Biological materials

Mutant

• MGNA-B336 (ykuF::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/1335 NBRP B. subtilis, Japan]
• BKE14060 (Δ[gene|CE990D33A12C50D22506A52E0099F7D97A762D4E|fadH]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE14060 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATGTTTATCCCTCCAATTG, downstream forward: _UP4_TAATCTGAATATTTATAAGT
• BKK14060 (Δ[gene|CE990D33A12C50D22506A52E0099F7D97A762D4E|fadH]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK14060 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATGTTTATCCCTCCAATTG, downstream forward: _UP4_TAATCTGAATATTTATAAGT

References

• 17189250,17919287